In vitro and In vivo Evaluation of a Plant Origin Acaricide and In vitro Evaluation of Plant Extracts Against Two-Spotted Spider Mite, Tetranychus urticae Koch

Dr. Bassam Fahed Al-Atalah, Mrs. Jihan Mahmoud Al-Abdulla, Eng. Nareman Mhana Al-Zugbi

Abstract


This study was performed at Sweida Research Center/GCSAR/ in Syria during 2016. The effect of garlic and soft wheat crude extracts as well as the effect of matrine (plant-based acaricide), jolly and sanmite (chemical-based acaricides) were tested under in vitro conditions on two-spotted spider mite, Tetranychus urticae Koch (TSSM). In addition, acaricides used were evaluated under in vivo conditions. Survival % of TSSM was calculated, neglog transformed and subjected to ANOVA analysis using Tukey HSD test. In vitro bioassay results revealed a significant effect of garlic and wheat extracts as well as matrine, jolly and sanmite. After 72 hours, no difference was observed between plant extracts, and among tested acaricides. In vivo bioassay results also showed a significant effect of acaricides used where jolly was the best and matrine and sanmite were comparable. These findings support the idea of using plant-based acaricides (including lectin-based acacicides) as an alternative strategy of using chemical-based acaricides. Taking into account the advantages of in vitro bioassays and based on the results of this study, we suggest predicting in vivo response from in vitro results although this issue needs to be tested first for the crude plant extracts to evaluate their stability under in vivo conditions.

 

 

DOI: 10.33977/2106-000-003-003


Keywords


Spider Mite, Plant Extract, Matrine, Survival %, Lectin.

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