Assessment of Genetic Relationships in Some Syrian Pistachio Cultivars and Genotypes, Pistacia vera L., Based on ISSR Markers

Najwa Motaeb Alhajjar, Bayan Mohammad Muzher

Abstract


It was clearly observed that the performance of the commercial pistachio genotypes was confusing within each variety according to the accredited substantial criteria of international descriptors. Therefore, the current work aimed to assess the genetic variation among 10 genotypes and cultivars, including 4 Ashouri, 2 Batouri, Ajami, Beid alhamam, and Ras alkharof across fifteen ISSR primers in Sweida Research Center 2019-2020. All of the used primers were polymorphic, which revealed a total of 148 bands, 141 of them were polymorphic (95.27%). The number of bands ranged from 6 to 15, with an average of 9.87 bands for each locus. Genetic similarity among all studied genotypes and cultivars ranged from 0.31 to 0.73. Depending on the UPGMA algorithm and the Dice equation, the cluster analysis divided the studied material into three main clusters. The first and second clusters comprised the following white genotypes: White Batouri, Batouri cultivar, Grahi, Beid alhamam, and Ras Alkharof that are analogous in many morphological characters, and the third cluster contained all other genotypes and cultivars: Ajami, White Ashouri, Ashouri cultivar, Ashouri Abureha, and Ashouri Mawardi. The current results demonstrated the efficiency of the ISSR technique by revealing the genetic variation among P. vera genotypes and cultivars and separating all of them into standing apart clusters according to their resembling appearance.


Keywords


Pistacia vera L., genotypes, ISSR, genetic similarity, clustering.

Full Text:

PDF

References


- Alhajjar, N.M., Hamed, F. & Muzher, B.M. (2017). Genetic similarity among pistachio (Pistacia vera L.) female genotypes and cultivars planted in Sweida province using SSR technique. Damascus Journal of Agricultural Science, 33(1), 239- 256.

- Alhajjar, N.M. & Muzher, B.M. (2017). Identification of male genotypes in Pistacia vera L. species using SSR markers. International Journal of Environment, 6(2), 1-12.

- Alhajjar, N.M., Muzher, B. M. & Hamed, F. (2016). Assessing genetic relationships between Pistacia vera L. Hybrids and their parents (P. vera × monoecious genotypes of Pistacia atlantica) using SSR markers. Jordan Journal of Agricultural Science, 12(1), 148- 157.

- Alhajjar, N. M., Muzher, B. M. & Hamed, F. (2015). The effect of pollen grains of Pistacia vera and Pistacia atlantica (unisexual and hermaphrodite) on quality parameters of Ashouri and Batouri pistachio cultivars. Jordan Journal of Agricultural Science, 11(1), 15- 25.

- Al-Saghir, M.G. & Porter, D.M. (2012). Taxonomic revision of the genus Pistacia L. (Anacardiaceae). American Journal of Plant Science. 3, 12- 32.

- Amirebrahimi, F.F., Meimand, M.M., Karimi, H.R., Malekzadeh, K. & Yajabadipour, A. (2017). Genetic diversity assessment of male and female pistachio genotypes based on ISSR markers. J Plant Mol Breed, 5(1), 31- 39.

- Baghizadeh, A. & Dehghan, E. (2018). Efficacy of SCoT and ISSR markers in assessment of genetic diversity in some Iranian pistachio (Pistacia vera L.) cultivars. PHJ., 1(1), 37- 43.

- Dice, L. R. (1945). Measures of the amount of ecological association between species. Ecology, 26(3), 297- 302.

- Ehsanpour, A.A., Tavassoli, M. & Arab, L. (2008). Sex determination of Pistaia vera L. using ISSR markers. Malays. Appl. Biol., 37(2), 25-28.

- Esfandiyari, B. & Davarynejad, G.H. (2011). Data to sex determination in Pistacia species using molecular markers. Euphytica. DOI 10.1007/s10681-011-0527-6.

- Fares, K., F. Guasmi, L. Touil, T. Triki & Ferchichi, A. (2009). Genetic diversity of pistachio tree using Inter-Simple Sequence Repeat markers ISSR supported by morphological and chemical markers. Biotechnology, 8(1), 24-34.

- Ghorbel, A., Ben Salem-Fnayou, A., Chatibi, A. & Twey, M. (1998). Genetic resources of Pistacia in Tunisia. In: Towards a comprehensive documentation and use of Pistacia genetic diversity in Central and West Asia, North Africa and Europ. Padulosi, S. and Hadj-Hassan, A. (eds.). IPGRI Report of the IPGRI Workshop, 14-17 December, (P: 62-71).

- Ghorbel, A. & Kchouk, M. L. (1996). Genetic resources of horticultural crop in Tunisia. Second Meeting of the WANA Working Group on Horticultural Crop. International Plant Genet.Resour. Inst., Aleppo, Syria.

- Kafkas, S. & Topaktas, M. (2003). Chromosome numbers of Four (Anacardiaceae) species. Journal of Horticultural Science Biotechnology., 78, 35–38.

- Kafkas, S., Ozkan, H. B., E., Acar, I., Atli, H.S. & Koyoncu, S. (2006). Detecting DNA polymorphism and genetic diversity in a wide germplasm: comparison of AFLP, ISSR, RAPD markers. American Society for Horticultural Science, 131, 522-529.

- Kamiab, F., Ebadi, A., Panahi, B. & Tajabadi, A. (2014). RAPD analysis for sex determination in Pistacia vera L. Journal of Nuts, 5(1), 51-55.

- Kebour, D., Boutekrabt, A. & Mefti, M. (2012). Using Inter Simple Sequence Repeat (ISSR) markers to study genetic polymorphism of pistachio (Pistacia vera L.) in Algeria, African Journal of Biotechnology, 11 (29), 7354- 7360.

- Mahmoodnia, M. & Malekzadeh, K. (2017). Genetic diversity assessment of male and female pistachio genotypes based on ISSR markers. J. Plant Mol. Breed, 5(1), 31-39.

- Noroozi, Sh., Baghizadeh, A. & Javaran, M.J. (2010). Study on genetic diversity of some Iranian pistachio (Pistacia vera L.) cultivars using RAPD, ISSR and SSR markers: Amplification study. Biotechnology, 4(3), 120-125.

- Noroozi, Sh., Amin, B. & Javaran, M. J. (2009). The genetic diversity of Iranian pistachio (Pistacia vera L.) cultivars revealed by ISSR markers. BioDiCon, 2(2), 50- 56.

- Porebski, S., Bailey, G.L. & Baum, B.R. (1997). Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components.Plant Molecular Biology Reporter, 15 (1): 8-15.

- Tagizad, A., Ahmadi, J., Haddad, R. & Zarrabi, M. (2010). A comparative analysis of ISSR and RAPD markers for studying genetic diversity in Iranian pistachio cultivars. Iranian Journal of Genetics and Plant Breeding, 1(1), 6-16.

- Turhan-Serttas, P. & Ozcan, T. (2018). Intraspecific variations studied by ISSR and IRAP markers in Mastic tree (Pistacia lentisecus L.) from Turkey, Trakya University. Journal of Natural Science, 19(2), 147-157.




DOI: http://dx.doi.org/10.33977/2106-000-005-003

Refbacks

  • There are currently no refbacks.


Copyright (c) 2022 Palestinian Journal of Technology and Applied Sciences (PJTAS)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.